Instructor: Learntoupgrade & LSSSDC Certified
Language: English & Hindi
Validity Period: Lifetime
Genetic Engineering Using CRISPR Technology
Genetic engineering (also called genetic modification) is a process that uses laboratory-based technologies to alter the DNA makeup of an organism. This may involve changing a single base pair (A-T or C-G), deleting a region of DNA or adding a new segment of DNA. For example, genetic engineering may involve adding a gene from one species to an organism from a different species to produce a desired trait. Used in research and industry, genetic engineering has been applied to the production of cancer therapies, brewing yeasts, genetically modified plants and livestock, and more.
Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating or copying the genetic material of interest using recombinant DNA methods or by artificially synthesizing the DNA. A construct is usually created and used to insert this DNA into the host organism. The first recombinant DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40 with the lambda virus. As well as inserting genes, the process can be used to remove, or "knock out", genes. The new DNA can be inserted randomly, or targeted to a specific part of the genome.
Importance of Genetic Engineering
Genetic modification allows changes in genes to transfer genetic material in different organisms. It is possible to introduce foreign genes into crop plants and express these in specific tissues. Transfer of genes can help to improve crop protection. Genetic modification can be done with various viral and non viral methods. Direct manipulation of DNA sequences can lead to different expression levels. Choosing the best method for gene delivery can be a difficult task, particularly to transfer genetic material in a proper way. This review highlights, basic mechanism and importance and different methods used for efficient gene delivery. Hence, understanding of intracellular machinery plays a crucial role for designing a more effective gene delivery environment.
Jobs directly related to your degree include:
Jobs where your degree would be useful include:
Salary Range Provided in Genetic Engineering field:
Candidate in jobs or training period ranges from 2-2.8 Lakh per annum
For research associate, it ranges from 3.5 lakh to 5 lakh per year.
Junior scientists vary from 6-9 lakh per annum
Whereas for a senior scientist, it varies between 15-16 lakh per annum in private companies.
Not only in India but in foreign also if you are looking for a career the average annual base salary is $159,339 to $194,895 of geneticists in the USA. For example, a doctoral researcher gets a package of more than 22 lakh per annum (i.e. € 29,060/ year) whereas for postdoctoral researchers more than 50 Lakh per annum (€50,525/year). As the experience in this field increases, the salary packages offered in both private and government sector increases.
Who can enroll?
Students pursuing their Life Science / Biotechnology / Bioscience / BSc / BTech / MSc / MTech who aspire to work in the clinical research field.
o Frequently Asked Questions:
o What is the time limit to complete the course?
Unlimited access until you decides to complete the course. But once you have given your certification test, the course ends. If you fail in the test, you can revise the course again
o How do I evaluate myself?
Time to test all the knowledge you have gained. Take the test at the end of the course and evaluate yourself. Every Learning Course has a test at the end.
o How to get the certificate?
To avail, the certificate, take the test at the end of the course. Clearing the test (with a minimum of 50%) will earn you the certificate. The certificate can be downloading from our website. The certificate has a unique code by which anybody can verify the certificate on our website.
What Benefits are you going to get from this course?
|Secret Of DNA and its Manipulation|
|Module 1. Introduction to DNA|
|1. Introduction to DNA|
|Module 2. Structure of DNA|
|Module 3. Organization of DNA into chromosomes|
|Organization of DNA into chromosomes|
|Levels of DNA Packaging|
|Significance DNA Packaging|
|Grooves of DNA|
|Module 4. Mitochondrial DNA|
|Introduction To Mitochondrial DNA|
|Module 5. Introduction to DNA Manipulation or Genetic Manipulation|
|5.1. Genetic Manipulation|
|5.2. Type and Advantage of Genetic Manipulation|
|Module 6. Recombinant DNA Technology|
|6.1. Introduction To Recombinant DNA Technology|
|6.3. Steps in rDNA Technology|
|Module 7. DNA Libraries|
|7.1. DNA Library|
|7.2. cDNA Library|
|Module 8. Gene/ Genetic Editing|
|8.1. Introduction to Gene Genetic Editing|
|8.2. Types of Gene Genetic Editing|
|Module 9. Zinc-finger nucleases (ZFNs)|
|9.1. Introduction of Zinc-finger nucleases (ZFNs)|
|9.2. Domains of Zinc-Fingure Nucleases(ZFNs)|
|9.3. Steps in ZFNs|
|9.4. Applications of Zinc-finger nucleases (ZFNs)|
|Module 10. Transcription Activator-Like Effector Nucleases (TALENs)|
|10.1. Introduction to TALENs|
|10.2. Steps in TALENs|
|10.3. Applications in TALENs|
|Module 11. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)|
|11.1 Introduction CRISPR- Cas9|
|11.2. CRISPR- Cas9 Genome Editing Tool|
|11.3. Structure of CRISPR- Cas9|
|11.4. History of CRISPR - Cas9|
|11.5. Mechanism of CRISPR Immunity|
|11.6. Types of CRISPR Cas|
|11.7. Steps in genome editing using CRISPR- Cas9|
|11.8. Application of CRISPR- Cas9|
|11.9. Challenges for CRISPRCas9 Application|
|Module 12. Applications of Genetic Manipulation|
|12.1. Introduction to GMOs|
|12.2. History of GMOs|
|12.3. Applications in GMOs|
|12.4. Controversy in GMOs|
|12.5. Gene Therapy|
|12.6. How Does Gene Therapy Work|
|12.7. Is Gene Therapy Safe|
|12.8. CAR T Cell Therapy, RNA Therapy, and Other Genetic Therapies|
|12.9. Origin of Gene Therapy|
|12.10. Ethical Issues Surrounding Gene Therapy|
|Module 13. Future of Genetic Manipulation|