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Oligonucleotide Microarrays in Microbial Diagnostics

Date:- 16th August 2022 

Oligonucleotide Microarrays
Oligonucleotide microarray (DNA chip)-based hybridization analysis is a promising new technology which potentially allows rapid and cost-effective screens for all possible mutations and sequence variations in genomic DNA.
Oligonucleotide microarrays offer a fast, high-throughput alternative for the parallel detection of microbes from virtually any sample. The application potential spreads across most sectors of life sciences, including environmental microbiology and microbial ecology; human, veterinary, food and plant diagnostics; water quality control; industrial microbiology, and so on. The past two years have witnessed a rapid increase of research in this field. Many alternative techniques were developed and validated as seen in 'proof-of-concept' articles. Publications reporting on the application of oligonucleotide microarray technology for microbial diagnostics in microbiology driven projects have just started to appear. Current and future technical and bioinformatics developments will inevitably improve the potential of this technology further.

What are oligonucleotides used for?
For most uses, oligonucleotides are designed to base-pair with a strand of DNA or RNA. The most common use for oligonucleotides is as primers for PCR (polymerase chain reaction). Primers are designed with at least part of their sequence complementary to the 5' end of the sequence targeted for amplification.

What are the types of oligonucleotides?
Oligonucleotides are short, single- or double-stranded DNA or RNA molecules, and include antisense oligonucleotides (ASO), RNA interference (RNAi), and aptamer RNAs. ASO and RNAi oligonucleotides are intended mainly for modulating gene and protein expression.

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